Antibody Analysis
M-Scan's analytical laboratories provides a full antibody analysis package, tailored to the FDA and ICH guidelines and available to GLP/GMP, including:- Amino acid sequence analysis, using automated sequencing via Edman degradation and/or MS/MS sequence analysis- Amino acid composition analysis to provide protein concentration, extinction coefficient and/or molar amino acid ratios- N-Terminal and C-Terminal amino acid sequence analysis, using automated Edman chemistry and/or mass-spectrometric mapping/sequencing strategies- Peptide MS Mapping analysis, using digestion and MS strategiesDisulfide bridge analysis, using MS and MS/MS- Carbohydrate structure, including monosaccharide composition analysis, oligosaccharide population screening by HPAEC-PAD and MS, linkage analysis and glycosylation site determination- Determination of intact molecular weight- Electrophoretic and isoform patterns, including CE- Liquid chromatographic patterns, including RP-HPLC, SEC and IEX- Identification of post-translational modifications- Spectroscopic profiling, using CD- Identification of product related impurities, including degradation products (deamidation, oxidation, mis-matched S-S bridge forms)- Protein aggregation assessment by AUC and SEC-MALLS- NMR is available at research levelOur antibody analysis service can be tailored to the requirements of the client - selected individual analyses or the "full antibody analytical package" can be performed.